yngB [2019-08-01 14:31:46]

similar to UTP-glucose-1-phosphate uridylyltransferase

locus: BSU_18180

pI: 5.20

mw: 32.99 kDa

protein length: 297 aa

gene length: 894 bp

function: unknown

product: unknown

essential: no

synonyms

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.6|Cell wall/ other/ based on similarity]

Gene

Coordinates: 1,946,702 1,947,595

The protein

Protein family

• UDPGP type 2 family (with [protein|D368988F85D8436DD424B0B4A1591BDF092A8EA7|GtaB] and [protein|FC455B727F66632D94D93E103B30748944ED04A0|YtdA], according to UniProt)

Paralogous protein(s)

• [protein|D368988F85D8436DD424B0B4A1591BDF092A8EA7|GtaB], [protein|FC455B727F66632D94D93E103B30748944ED04A0|YtdA]

Structure

• [PDB|5I1F] (from Burkholderia vietnamiensis, 50% identity)

Expression and Regulation

Operons

• genes: [gene|CBCBF948979D9D63CBA86A9ED865E43B1F85A0AD|yngA]-[gene|69C5D9EF7ED43BCAB7A9D6148EDD323CE72F8E3D|yngB]-[gene|289828D37CF5422908316ED2EF1AEAE76F84161D|yngC]
  description: [Pubmed|20512483]

  sigma factors

  • [protein|081DF3EE9FA56209D648C7677188C61CE3AA8E41|SigM]: sigma factor, [Pubmed|18179421], in [regulon|081DF3EE9FA56209D648C7677188C61CE3AA8E41|SigM regulon]

  regulatory mechanism

  • [protein|706983E6942E883D3A9D45693E7B4015AEABE60B|YclJ]: activation, [Pubmed|20512483], in [regulon|706983E6942E883D3A9D45693E7B4015AEABE60B|YclJ regulon]

  regulation

  • expression during spore [SW|germination] is strongly reduced under conditions of osmotic stress [Pubmed|27766092]

Biological materials

Mutant

• MGNA-B396 (yngB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1395 NBRP B. subtilis, Japan]
• BKE18180 ([gene|69C5D9EF7ED43BCAB7A9D6148EDD323CE72F8E3D|yngB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE18180 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TCTCACTTTTTTTCTCATTC, downstream forward: _UP4_TAAAAAAATTGTAACCATCT
• BKK18180 ([gene|69C5D9EF7ED43BCAB7A9D6148EDD323CE72F8E3D|yngB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK18180 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TCTCACTTTTTTTCTCATTC, downstream forward: _UP4_TAAAAAAATTGTAACCATCT

References

• 20512483